Exact(1)
The primer bases should only be removed immediately before the variant calling step to ensure that the variants can be called irrespective of where they occur within the amplicon insert region.
Similar(59)
This month the energy secretary, Ed Davey, said the government was looking to introduce a single emergency number that people could call irrespective of where they live to report power cuts.
"In particular, people need to know how to contact the network operator in their region if there is a power cut, and we're looking to introduce a single emergency number which people can call irrespective of where they live.
When the safety aspect of the product is brought into question as a result of a change, a prior approval supplement is called for, irrespective of the suggested filing category for that change.
"Nevertheless, we recognise that all calls, irrespective of the purpose, should be made in the right way and we accept that our processes, in place at the time of the review by Ofcom, were inadequate.
The logical but somewhat extreme viewpoint taken in this manuscript, in which transmission units are called evolutionary units (irrespective of selection units), appears to me not devoid of problems.
It is home only to regulars and locals and the barmaid calls you "dear" irrespective of sex or age.
It is conventional to describe drag forces in terms of a dimensionless quantity called the drag coefficient; this is defined, irrespective of the shape of the body, as the ratio [FD/(ρv02/2 A] and is denoted by CD.
SNPs for which genotypes were called in at least 9 turkey populations (irrespective of whether SNPs were segregating in all these populations) were selected and utilized for the genetic diversity analysis.
It said giving it to schools irrespective of performance was flawed and called for a portion of the cash to be ring-fenced for schools that boost pupils' exam results and job opportunities.
In this context, synthetic chemist invented simple easy reaction called click chemistry with high substrate tolerance reaction irrespective of the environment to address multidisciplinary drug development.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com