These have landed him in what we could generously call difficulties.
However, when the target HPV DNA at the priming sites is less preferred by the primers and is amplified with lower efficiencies, the poorly used primers may bind to other competing non-HPV DNAs, causing nonspecific co-amplification during PCR thermocycling, which may in turn generate base calling difficulties in DNA sequencing.
In his work Foundations of Empirical Knowledge (1940), Ayer called this difficulty "the egocentric predicament".
I once wrote an essay called The Difficulty of Imagining Other People, in which I argued that the ease with which we injure people directly corresponds with the difficulty we have imagining them and their feelings.
Pocket Gamer Mark Brown called its difficulty "steeper than the Alps".
This is perfectly possible, especially when you consider that Naughty Dog implemented something called Dynamic Difficulty Adjustment within Crash Bandicoot.
We also created a ratio called the difficulty ratio - the ratio of AP/IB tests divided by the total offered.
The item characteristic β j, called "item difficulty" in the classical Rasch example, in this context represents the item propensity to obtain, by the respondents, systematically high or low scores when measuring the latent trait of interest.
The explicit feedback reported by the subjects in their questionnaires also allowed us to analyze the difficulty and time required to solve each questionnaire item, which we called task-difficulty and resolution-time respectively (see Sections 5.1.4 and 5.2.3).
He called the difficulties for social media on the trail "a bummer".
Moreover, Churchill assigned refugee rescue matters to his Foreign Office, which repeatedly sabotaged rescue opportunities because it feared what one official called "the difficulties of disposing of any considerable number of Jews should they be rescued from enemy occupied territory".
