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From both signals we extracted stimulus-aligned epochs that ranged from 20 ms before call onset to 20 ms before the next call onset.
Of the strongly modulated neurons, we observe a range of responses: most cells displayed early (<25 ms after call onset) increases in firing rates, while a few late responders (>50 ms after call onset) were also observed.
Analysis of tracked whiskers in the high-speed videos relative to call onset shows that the temporal coordination of calling and whisking is indeed observable (Video 1).
The number of calls in the first 0.5-s bins (Nbase) served as baseline and those in the 0.5-s bins after call onset (Nresponse) as the response.
50 ms after call onset were removed from the analyses to avoid crosstalk (i.e., in case the same birds had been recorded by both backpacks).
In addition, a few late responders were also observed (response latency >50 ms after call onset; Figure 3 figure supplement 2E).
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Analysis of PSTHs triggered to the call onsets revealed the presence of several neurons in the auditory cortex that responded strongly to the calls vocalized within that session.
(C ) Distribution of call onsets were significantly non-uniform (Hodges Anje test) relative to whisking cycle of emitter itself (top) but not for the interacting partner (bottom).
Using the temporal information encoded in call onsets, we found that the timing of calls was not random but instead occurred in significant vocal interactions between individuals of social groups.
Such rhythmicity was not obvious when the call onsets across interaction partners were analyzed ('cross', Figure 2E, right), suggesting that the coordination of whisking and vocalization is restricted to individuals.
We observed that the relationship of call onsets to whisking phase was distributed significantly non-uniformly relative to whisking for call emitter (n = 664, p = 0.0014, Figure 2C, top, Hodges Anje test) but not for interacting partner (n = 705, p = 0.06, Figure 2C, bottom).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com