Occasionally, more than one base call was detected in a minor population of reads.
In pool 66B, a variant A call was detected in 6 reads of a total of 2999 reads (greyed box), for an F = 1 498.
We also demonstrate that subunits derived from Norwegian stereotyped calls were detected in some Norwegian variable (nonstereotyped) calls as well as the stereotyped calls of other killer whale populations.
No differences in patterns of calling were detected in a comparison of male and female pups, therefore data were collapsed across sex.
USV calls were detected and counted for 5 min with a bat detector (Mini 3 bat-detector, Ultra Sound Advice, U.K). at 70 kHz.
Surprisingly, between 1 and 5 percent of the SNP that produced genotype calls were detected as polymorphic in these outgroup species, despite the fact that only 2 or 4 individuals were genotyped within each outgroup species.
Ten specific categories of calls were detected, designated as complex, harmonics, two-syllable, upward, downward, flat, chevron, short, composite and frequency steps, consistent with previous reports [21], [22], [24], [54].
113 of the 116 calls were detected by Sanger sequencing.
All 56 previously identified variant calls were detected and no false positive calls were assigned.
