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The 454 sequencing method does not call bases directly but nucleotide flows are indicated by a light signal.
Phred and Phrap were used to call bases and produce an alignment (∼10×) and Consed was used for manual verification of quality [30], [31], [32].
Chromatograms were reanalyzed using PHRED v0.020425.c [36], [36] using the phredPhrap Perl script supplied with the CONSED distribution to call bases and assign quality scores.
PHRED software was used to call bases for the sequencing of the LongSAGE tags[ 33, 34].
PineSAP implements a combination of Phred, Phrap and ProbconsRNA to efficiently and accurately call bases and align re-sequencing reads.
Sequence data in the chromatogram file were processed using Phred [ 53] to call bases with cutoff value >20 and Crossmatch to mask vector sequences.
Similar(48)
On what information was this call based?
A gut call, based on preference more than assurance.
And Joyce made a call based on previews -- which I was told you don't do.
It's a judgment call based on research and wind conditions and safety.
Her way was to issue a rallying call based on discussing immigration and growing economic inequality.
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