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Arterial blood gas samples were analyzed using a CO-oximeter (ABL725, Radiometer, Copenhagen, Denmark) that was calibrated daily by laboratory staff and has a 2-hourly automatic internal calibration sequence.
An ingenious new calibration sequence guides you through adjusting your set for the best picture.
At the older end of the calibration sequence the Sun, at 4.6 billion years, has a well known rotation period.
After initialization, the program runs through the calibration sequence which sets the boundary of the playing field.
In addition, each laboratory has developed its own strategy of analysis on calibration, sequence design, standards utilisation and data treatment without a clear consensus.
Assume that known calibration sequence observations are used for each of these signals.
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Orange boxes show expected F-tRNA pulses in calibration sequences.
However, it is essential to critically evaluate the impact of methodology and other parameters (taxon sampling, fossil calibrations, sequence regions) on the reliability of results.
As a calibration point, sequence data from R. rattus and M. musculus, with a divergence date estimated at 12 Mya based on fossil records, was used [ 101].
P. rapae and its congener P. brassicae diverged approximately 11.75 Myr ago, based on temporal calibration of sequence divergence in the EF-1α gene as previously applied to Pieridae [ 4].
A conventional calibration of mtDNA sequence divergence indicates a mid to late Pliocene evolution of the main clades in Lophura, which could have diversified in allopatry in continental South East Asia.
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