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The magnification at the CCD camera was calibrated using standard calibration line and cross grating replicas with 1200 and 2160 lines mm 1 for low and intermediate magnifications, respectively.
In order to obtain a calibration line, microelectrodes were calibrated at the beginning and end of each experiment using standard solutions covering the experimental range of each ion.
Before, the sensor was conditioned for 20 loading cycles and calibrated with a 2-point calibration line computed by the measurement software (I-Scan 6.1).
Calibration points achieved by CRMs and aqueous solutions are covered by one calibration line, indicating the absence of matrix effects.
The calibration line of filtrate absorbance versus analytical concentration was linear over two orders of magnitude between 0.4 and 40 ppm TNT in final solution.
as standard solution was employed to establish the calibration line.
The small values of the figures indicate low scattering of the points around the calibration line.
The amount of free DOX was calculated according to the calibration line.
For all experiments done in LRW solution, calibration line was done with LRW.
Figure 3 qPCR calibration line of Methanospirillum hungatei JF1 in the presence of HS-DNA.
Except for one student, all could determine the calibration line (regression line).
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