Figure 2 shows our calibrated voltage-frequency levels, and the one provided in RCCE source code.
Electrical resistance was obtained by impressing a family of voltage step across the membrane (30 mV/2 s) and calculated according to the ohm's law RM = (EM/Ei−EM *Ri where Ri is the series resistance (stated at 1 MΩ, which is the resistance of a glass pipette), Ei is the calibrated input voltage, and EM is the voltage across the bilayer.
Through experimental evaluation, we have calibrated the voltage and frequency pairs that works for our SCC unit.
The coldwire is calibrated from voltage to degrees Celsius in post-flight analysis using a co-located commercial sensor (ADS1118) that has much slower frequency response than the coldwire (time constant about 2 s at 14 ms−1 airspeed) but has a specified accuracy better than ±1 °C over −40 to +125 °C range (Table 2).
In this paper, we present adaptation dynamics of photoreceptor and LMC output of WT flies at three luminance levels: dim (1,850), middle (60,000) and bright (1.85×106 photon/s), as calibrated from voltage responses of photoreceptors to single photons [25]; see also (Fig. S1).
The expanded uncertainties of the calibration of the 10 1 and 100 1 ratios are respectively 4.6 × 10−7 and 6.6 × 10−7, suitable to employ the divider in an automated measurement setup with a DMM and a DC voltage standard to calibrate DC voltage generators as top-level calibrators, widespread in secondary electrical laboratories.
At National Institute of Metrological Research (INRIM), an automated setup to calibrate dc voltage generators, mainly top-level calibrators, from 1 mV to 1 kV has been developed.
In order to calibrate the voltage response of the fluorescent sensor in our experimental conditions (see Methods), we loaded organotypic cultures from wild type mice, euthanized at postnatal day 5 (P5), with Vf2.1.Cl.
While RCCE source code only provides two usable voltage levels, our calibrated profile provides four voltage levels.
The digitiser is also routinely calibrated against a known voltage.
The output signal was then calibrated to translate measured voltage to radioactivity concentration.
