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Second, a deflection-to-diode voltage calibration was carried out by translating a rod attached to the wire using a micromanipulator equipped with a calibrated micrometer.
Each of the aortic rings was incubated in 2 mL DMEM medium containing either vehicle (dH2O) or Ang II (100 nmol/L) for 60 min. The inner diameters were calculated by measuring luminal diameter through the transverse section of the thin-vessel ring by using a calibrated micrometer eyepiece.
Measurements were made using the calibrated micrometer of a Nikon inverted microscope.
The sarcomere length in our setup was verified by a calibrated micrometer for several bundles from adult and senescent rats under a 400× Zeiss lens.
The cannulae were attached to two calibrated micrometer driven 5 μl Hamilton syringes (Hamilton Company, Reno, NV, USA) via a 250 μm inner diameter Tygon tube (Saint-Gobain, Akron, OH, USA).
The injections were made using pressure over a period of 5 to 10 seconds and were monitored by use of a dissecting microscope with a calibrated micrometer disk.
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Ten measurements were taken at even intervals around each airway cross-section using an Openlab camera-calibrated micrometer.
A total of 100 microspheres were measured for particle size using a calibrated ocular micrometer at 100× objective and mean diameter was reported.
Nuclei counts were made from slides using a calibrated Filar micrometer eyepiece (Reichert Scientific Instruments, Buffalo, NY).
The morphometric details were recorded with the help of a calibrated ocular micrometer (Erma, Tokyo).
As an indicator of wear, linear femoral head penetration was assessed directly using a calibrated digital micrometer (accuracy, 0.001 mm).
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