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All 61 internal duplicate samples showed complete concordance of integer copy number, and an average calibrated copy number was generated from all PRT systems.
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Notably, the paralog ratio test (PRT), which uses paralogous, copy-number-invariant sequences elsewhere in the genome as embedded controls to carefully calibrate copy number measurements [ 59], appears to produce highly accurate copy number measurements.
We were able to initially calibrate copy numbers using segregation in 16 three-generation CEPH pedigrees, for many of which only a single integer solution was consistent with the data.
The 2−ΔΔ CT method was used to normalize and calibrate CYP51 copy numbers as described in [ 98].
As the "T1 + 829 amols" sample added peptide calibrated to 100 copies per cell, both the y7 and b6 MS spectra indicate 9.8 copies of GILGFVFTL per cell is loaded on T1 cells by incubation in a solution of peptide at 62 pg/mL.
Detection of individual genotypes was calibrated against known copies of cloned plasmids.
The team then uses quantitative sandwich ELISAs that are calibrated to measure the copy number of proteins per cell.
The average CT from 28 DAP measurements were calibrated as 100 or 1000 copy numbers, and the relative copy numbers of a gene were averaged over triplicates.
Now in the final year of his Ph.D., Talboys joined the SRC in 2002 to work on calibrating a spare copy of the XRS in the lab, so that the whole range of new conditions Beagle 2 would have encountered on Mars would not have affected the results.
Quantification of IS900 copy number was calibrated using a 10 fold dilution curve of pIDL60 stock solutions.
The sites were calibrated using samples with normal copy numbers (Supplemental Material) and then adjusted for batch effects (Supplemental Material).
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