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The model is calibrated by assuming that the current European map is stable.
Divergence times were calibrated by assuming of a mid-Cretaceous (~100 MYA) origin of Neoaves.
The radial tonometric waveform was calibrated by assuming equivalence of mean [(2*Diastolic BP + Systolic BP /3] and diastolic brachial cuff pressure.
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Stock solutions of thiamine, TMP and TPP were calibrated by spectrophotometry assuming molar extinction coefficients of ɛ233 = 14.2, ɛ247 = 15.3 and ɛ247 = 13.0 (mmol/l)−1 cm−1, respectively [ 14].
To gain insight into the phylogeography of the region, we test a wide range of possible substitution rates in order to better date the African-Sicilian divergence of green toads: Based on only two haplotypes from Sicily, Stöck et al. [ 28] roughly calibrated their tree by "assuming that the last landbridge between North Africa and Sicily broke about 5.3 Mya".
Microscope was calibrated by green calibration slide before each experiment.
Open image in new window Fig. 5 Results of boundary shear stresses for large-scale compound channels (the solid line: k calibrated by Devi and Khatua approach, the dashed line: k assumed as Abril and Knight approach).
Open image in new window Fig. 3 Results of velocity simulations for large-scale compound channels (the solid line: k calibrated by Devi and Khatua approach, the dashed line: k assumed as Abril and Knight approach).
Since the compound channels considered here have a small width ratio, the k values calibrated by Devi and Khatua approach were higher than those assumed in Abril and Knight approach.
Open image in new window Fig. 2 Results of velocity simulations for small-scale compound channels (the solid line: k calibrated by Devi and Khatua approach, the dashed line: k assumed as Abril and Knight approach).
Open image in new window Fig. 4 Results of boundary shear stresses for small-scale compound channels (the solid line: k calibrated by Devi and Khatua approach, the dashed line: k assumed as Abril and Knight approach).
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