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Although CMIP5 results are not used to calibrate the method, CMIP5 spread is used to estimate the method uncertainty i.e. from the standard deviation of inferred versus calculated water cycle change in Fig. 4a.
To calibrate the method the PID signals are compared to quantitative data of individual tar compounds obtained by an established reference method.
Such an extensive validation exercise allowed us to prove that our approach is successful in estimating fatigue damage in aluminium welded details, resulting in predictions mainly falling within the two reference scatter bands adopted to calibrate the method itself.
For the LDC problem, results for Re= 1000 are compared with results using spectral methods in Botella and Peyret [O. Botella, R. Peyret, Benchmark spectral results on the lid-driven cavity flow, Comput. Fluids 27 (1998) 421 433] to calibrate the method in solving the steady state problem.
The experimentally proven miRNA-mRNA interactions from miRecords were sampled in order to calibrate the method.
The area of the corneas of untreated eyes was used to calibrate the method and determine the scaling factor.
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Calibrating the method with time periods that exhibit high variance of seepage fluxes yielded the best performance.
Resulting time series of flux dynamics are scaled to realistic absolute levels by calibrating the method with the areal water balance.
We calibrated the method using a set of manually-designed genomic probes that we have previously successfully employed for Southern blotting (see Table 1).
The presented results showed minor dependence on noise, spatial resolution, acquisition parameters and reconstruction settings for VOISchaefer, as was expected when calibrating the method.
Rest of the paper is organised as follows: Section 2 calibrates the methods undertaken for the study.
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