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This agenda applies to systems biology as a whole, and tools are becoming available to calibrate cell-level models (Bois 2009a; Vyshemirsky and Girolami 2008).
The assay was also able to detect at least 5 genome equivalents of cloned amplified target DNA, using purified DNA, or 7 colony forming unit (CFU) per reaction when using calibrated cell suspensions.
The calibrated cell feedback signal (microcalories per second) was collected at 2 s intervals.
Blood was obtained from a small incision made to a lateral tail vein and added directly into an EDTA containing vial and then diluted 1 1 with PBS and counted immediately using a Calibrated Cell Counter.
Oxygen data were not available for strain A. DOI: http://dx.doi.org/10.7554/eLife.08208.006 We then constructed the microbial community (MC) model by combining the three calibrated cell models into a community growing in a common substrate pool.
Meanwhile, the electrode was calibrated (in the calibration cell) and then moved to the experimental cell.
KCl is used to calibrate conductivity cell.
Tzero aluminum was used to calibrate the cell constant.
These pulling forces calibrate the cell's response to biological signals through mechanisms that remain unclear.
It is important to note that only data from monoculture experiments were used to calibrate the cell models for the three strains (A, SS and FS).
We calibrate these cell-specific models against experimental data describing the force deformation behavior of the same cell type, which were published by Peeters et al.
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