Previous research has established that adopters of most TMPPS are also adopters of most of the others, with Khanal et al. ([2010]) and Pruitt et al. ([2012]) showing this result for the U.S. dairy and cow-calf industries, respectively.
DVDs became the golden calf of the industry.
PC3 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Biological Industries, Beit-Haemek, Israel) supplemented with 10% heat-inactivated fetal calf serum (Biological Industries), 2 mM glutamine, and penicillin/streptomycin, at 37°C and 5% CO2.
Cells were cultured in RPMI (Roswell Park Memorial Institute) medium 1640+ GlutaMAX (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal calf serum (Biological Industries, Israel) and 1% penicillin-streptomycin (Invitrogen) in 5% CO2 at 37°C.
Murine neuroblastoma cells (ATCC number CCL-131; Neuro-2a or N2a) were maintained in Minimum essential medium (MEM) (GIBCO-BRL) supplemented with 10% fetal calf serum [Biological Industries, Israel], 2 mM L-glutamine [Sigma], 1mM sodium pyruvate [Sigma] and antibiotic-antimycotic solution (100× stock) [Sigma] at 37°C humidified incubator with 5% CO2.
The supernatant was removed into a 50 cc test tube containing foetal calf serum (Biological Industries, Beit Haemek, Israel).
Murine 3T3-L1 preadipocytes were cultured to confluence in Dulbecco's modified Eagle's medium (DMEM; Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA, USA) that was supplemented with 10% (v/v) calf serum (Biological Industries, Kibbutz Beit-Haemek, Israel).
HT29-Cl were grown in standard DMEM medium containing, 100 U/ml penicillin, 0.1 mg/ml streptomycin, 2 mM glutamine and 10% fetal calf serum (Biological Industries, Kibbutz Beit Haemek, Israel), in a 5% CO2 humidified atmosphere at 37 °C.
A2780 was cultured in RPMI (Roswell Park Memorial Institute) medium 1640 + GlutaMAX (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal calf serum (Biological Industries, Israel) and 1% penicillin-streptomycin (Invitrogen) in 5% CO2 at 37°C.
The parental lines were maintained in RPMI-1640 medium supplemented with 10% fetal calf serum (Biological Industries, Kibbutz Beit Haemek, Israel), 100 units/ml penicillin, 100 μg/ml streptomycin, and 25 μg/ml amphotericin B (Biological Industries, ATCC) at 37 °C in 5% CO2.
D122 cell cultures were maintained in DMEM supplemented with 10% heat-inactivated foetal calf serum (FCS, Biological Industries, Israel), penicillin (100 U ml−1), streptomycin (100 mg ml−1) (Biological Industries, Israel), 2 m M glutamine (Biolab Ltd. Israel), sodium pyruvate (1 m M) and nonessential amino acids (Biological Industries, Israel).
