Therefore, the multivariate approach elaborated in this study could be potentially used as a part of quality control programmes in the veal calf industry.
Previous research has established that adopters of most TMPPS are also adopters of most of the others, with Khanal et al. ([2010]) and Pruitt et al. ([2012]) showing this result for the U.S. dairy and cow-calf industries, respectively.
The parental lines were maintained in RPMI-1640 medium supplemented with 10% fetal calf serum (Biological Industries, Kibbutz Beit Haemek, Israel), 100 units/ml penicillin, 100 μg/ml streptomycin, and 25 μg/ml amphotericin B (Biological Industries, ATCC) at 37 °C in 5% CO2.
Cells were cultured in RPMI (Roswell Park Memorial Institute) medium 1640+ GlutaMAX (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal calf serum (Biological Industries, Israel) and 1% penicillin-streptomycin (Invitrogen) in 5% CO2 at 37°C.
Murine neuroblastoma cells (ATCC number CCL-131; Neuro-2a or N2a) were maintained in Minimum essential medium (MEM) (GIBCO-BRL) supplemented with 10% fetal calf serum [Biological Industries, Israel], 2 mM L-glutamine [Sigma], 1mM sodium pyruvate [Sigma] and antibiotic-antimycotic solution (100× stock) [Sigma] at 37°C humidified incubator with 5% CO2.
The supernatant was removed into a 50 cc test tube containing foetal calf serum (Biological Industries, Beit Haemek, Israel).
Murine 3T3-L1 preadipocytes were cultured to confluence in Dulbecco's modified Eagle's medium (DMEM; Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA, USA) that was supplemented with 10% (v/v) calf serum (Biological Industries, Kibbutz Beit-Haemek, Israel).
HT29-Cl were grown in standard DMEM medium containing, 100 U/ml penicillin, 0.1 mg/ml streptomycin, 2 mM glutamine and 10% fetal calf serum (Biological Industries, Kibbutz Beit Haemek, Israel), in a 5% CO2 humidified atmosphere at 37 °C.
A2780 was cultured in RPMI (Roswell Park Memorial Institute) medium 1640 + GlutaMAX (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal calf serum (Biological Industries, Israel) and 1% penicillin-streptomycin (Invitrogen) in 5% CO2 at 37°C.
DVDs became the golden calf of the industry.
D122 cell cultures were maintained in DMEM supplemented with 10% heat-inactivated foetal calf serum (FCS, Biological Industries, Israel), penicillin (100 U ml−1), streptomycin (100 mg ml−1) (Biological Industries, Israel), 2 m M glutamine (Biolab Ltd. Israel), sodium pyruvate (1 m M) and nonessential amino acids (Biological Industries, Israel).
