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Homeostasis model assessment of insulin resistance (HOMA-IR) values were calculated (HOMA calculator version 2.2.1) from fasting serum glucose and serum insulin levels to gauge the degree of insulin resistance.

FDR was calculated by BenjaminiHochberg.xlsx calculator Version 1.1 with a threshold adjusted P-value set at 0.05.

Insulin resistance (HOMA2-IR), insulin sensitivity (HOMA2-%S), and β-cell function (HOMA2-%B), were calculated using the HOMA calculator version 2.2 [ 29].

The homeostatic model assessment [ 15] was used to assess glucose handling and values calculated using the HOMA calculator, version 2.2 (© Diabetes Trial Unit, University of Oxford).

Based on the plasma glucose and serum C-peptide levels, the homeostasis model assessment for insulin resistance (HOMA2-IR) and insulin sensitivity (HOMA2 %S) values were calculated using the HOMA2 calculator version 2.2.3 [ 10, 11].

Based on updated homeostasis model assessment (HOMA) methods, HOMA insulin resistance (HOMA2-%IR) and HOMA β-cell insulin secretion (HOMA2-%B) were calculated by the HOMA2 calculator version 2.2 (http://www.dtu.ox.ac.uk/homacalculator/index.php) (29).

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Statistical analysis was done with one-way ANOVA using online calculator, Statistics Calculator version-3 beta, and the level of significance was set at p value <0.05.

EPV with confidence intervals was calculated using the EPV-Calculator version 1.12 [ 26].

HOMA estimates were calculated using specific insulin (SI) or radioimmunoassay (RIA) calculators (version 2.2).

(Statistics calculators, version 3.0).

Finally, we performed the post-hoc power analysis (Statistics calculators, version 3.0).

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