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Average nucleotide identity (ANI) was calculated using ANI calculator (http://enve-omics.ce.gatech.edu/ani/).edu/ani/
All those indexes were calculated using online biodiversity calculator (http://www.alyoung.com/labs/biodiversity_calculator.html).html
The free Ca2+ concentration was calculated using the Ca EGTA Calculator (http://maxchelator.stanford.edu/CaEGTA-TS.htm).
Using only evaluable PSA values, PSA-DT was calculated using an algorithm calculator (http://mskcc.org/applications/nomograms/prostate/PsaDoublingTime.aspx).aspx
The polymorphic information content (PIC value) was calculated using a PIC calculator (http://www.liv.ac.uk/~kempsj/pic.html) [ 24].
Ka/Ks ratios were calculated by the Ka/Ks calculator http://services.cbu.uib.no/tools/kaks and plotted on a tree using the methods of [ 53].
The duration of daylight at each sampling date and additional dates to build a photoperiod curve was calculated using the day length calculator (http://www.solartopo.com).solartopo.com
Protein half-lives were calculated using a web based calculator http://www.1728.com/halflife.htm.htm
GGD and DDH estimates were calculated using the GGDC 2.0 calculator (http://ggdc.dsmz.de) with default settings.
The DAS-CRP was calculated by a WEB-site calculator http://www.das-score.nl/dasculators.html.html
GC percentages of the introns were calculated with the internet tool GC calculator http://www.genomicsplace.com/gc_calc.html.com/gc_calc.html
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