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Body mass index (BMI) and SDS calculations were determined using the Epi Info nutrition calculator [33].
Power calculations were determined using power and sample size calculator software PS version 2.1.31 http://www.mc.vanderbilt.edu/prevmed/ps/.edu/prevmed/ps/
Statistical calculations were determined using the GraphPad Prism software package (GraphPad, Software, Inc., CA, USA).
NA inhibition assay and IC50 calculations were determined using nonlinear curve fit in GraphPad Prism version 5. Statistical analysis was conducted using either one-way or two-way ANOVA when appropriate.
Power analysis calculations were determined using PASS 2008 software package (NCSS, Kaysville, UT).
Sample size calculations were determined using Power Analysis and Sample Size software [ 38].
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The statistical significance of the difference between calculations was determined using one-way analysis of variance.
The sample size calculation was determined using data from a previous study examining plasma vWF concentration in elective open AAA repair patients [12].
This calculation was determined using equally efficient SYBR green qPCR primer sets as previously described [ 28].
After spot detection, automatic background correction, spot volume normalization and volume ratio calculation, dye ratios were determined using DeCyder software (GE Healthcare).
Calculation of IC50 values were determined using GraphPad Prism v3.02 (GraphPad Software, Inc .. Data from the cough studies are expressed as cough number due to either a capsaicin or a ovalbumin 4 minute exposure.
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