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For robustness calculations, concentration values of kinases and phosphatases were sampled in the range of 0.1-10 0.1-10times reference values (reference values are theiralues used foreferenceulations in the unperturbed system) and the sample parameter sets with their sampling ranges are givalues Additional file 2: Table S3.
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For calculations, concentrations below the LLOQ were set to ½ LLOQ (12.5 ng/L).
The diffusion equations used in the calculations of concentration profiles were those arising from irreversible thermodynamics and contain activity coefficients and cross-diffusional terms.
The calculation about concentration and thickness of film is performed using simulation of non-Rutherford backscattering (SIMNRA) software.
In order to perform these calculations, the concentrations of the ions in the NaCl solution and in seawater were determined using ICP-OES elemental analysis.
Further, the application of the Darken equation and the Leffler and Cullinan equation for the calculation of concentration dependence of diffusion coefficients in supercritical carbon dioxide is presented.
For all calculations, the concentrations and rates of Table 2 were chosen including those for k7, k8, and k−8.
Limits of Detection (LOD) were 31 ppb (dry weight) at IREC and 7.34 ppb (dry weight) at Aberdeen, and we used in our calculations all concentrations above the LOD from these two laboratories.
For calculations, radioligand concentrations were corrected for depletion.
Phantom studies resulted in the calculation of concentration values consistently within 4% of true values.
For the calculation of concentration, 1 gm of the tissue was assumed to occupy a volume of 1 ml.
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