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For cell doubling-time calculations, cells cultured thrice in triplicate were harvested and counted on each day of culture, and these cell numbers were used to determine the culture's doubling time.
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For DT calculation, cells were counted from P5 to P8. CaSR expression was evaluated on both large and small cell lines on day 4th after treatment.
For DT calculation, cells were seeded, at the density of 1000 cell/cm2, in the first well of a six well plate, counted every 48 hours and re-seeded in the next well of the same plate at the same density (1000 cell/cm2) to the end of the treatment.
From this, we propose a stereo matching calculation cell and a new H/W architecture.
In this paper, the driftage contained in each calculation cell is called a "segment," and the surface of the driftage contained in each cell is called the "segment surface" (see Fig. 4).
However, we lack comparisons of theoretical calculations of cell-to-cell variability in organelle abundances to experimentally measured organelle abundance distributions.
That is, in computer calculations the cells fire signals at certain rates, connect in conventional ways, squirt out familiar chemicals and behave just like real brain cells do.
For density calculations, adherent cells were dislodged from one well of a 6-well plate (9.6 cm2 per well), and suspended in 0.5 ml culture medium.
Calculations of cell seeding efficiencies were based on the number of cells that adhered to the mesh after culture for 30 min and for 6 h.
Figure 5 Direct contact measurements and calculations of cell viability.
Dose calculations of cell-free Hb were made as described above.
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