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The optimal design of pulse tube refrigerator can be greatly assisted by the enthalpy flow calculation with four dimensionless parameters introduced in this paper.

The distances to important amino acids are reported in Additional Fige 1: Table S1.

We took 4 and more as the number of multiplexes since, for the previous simulation, we made calculation with four multiplexes and in Serbia there is a trend of increasing number of multiplexes for national coverage.

The steepest descent direction and acceleration parameter are adopted for the calculation using one correction vector (N c =1), and seven randomly chosen linearly independent correction vectors are added to the steepest descent correction to create a calculation with eight correction vectors (N c =8).

The second half of the method is a similar calculation with two major differences.

All data with signal-to-noise ratios > 5 were acquired, and the peak area was used for peak calculation with zero level integration.

The conformational analysis and vertical excitation energies of carbazole-based molecules were carried out by B3LYP calculations with three basis sets: 6-31G, 6-31G(d) and 6-311G d 6-311G d

Description of π π interactions of various conformations in the vicinity of the conformational defect is done through evaluation of phenyl angle-distance plot obtained from optimized geometries and molecular energy evaluation obtained from single point calculations with three different density functionals.

It is clear that the result of any calculation performed with three parameters that have a significant associated error will itself have an associated error, and an important question is thus whether the error in the results will still permit the drawing of relevant conclusions.

Further, the hydrodynamic interaction coefficient was computed via (i) dilute limit calculations (with two different forms for the radial distribution function) or (ii) a combination of perturbation theory and the results of an exact numerical solution for an ordered system.

For its practical application, it is necessary to study the influence of the depth and cross-section of the tunnel and to confirm the calculations with two- or three-dimensional simulations carried out with finite elements or explicit finite differences programs.

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