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The Temperature for Free Energy Calculation was set to 37 °C to standardize with other programs that offer a static 37 °C without the option to alter the temperature parameter.
The temperature for ΔG calculation was set to 37 °C under Preferences > Dimers to standardize with other programs that offer a static 37 °C without the option to alter the temperature parameter.
Time window for each calculation was set to 400 s.
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The discount rate applied for the annuity calculation was set to 3.5% for annual crops as well as for SRC.
The region for the cross-correlation calculation was set to the 25 ms center region of the frame, where the signals are more stable.
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The electron temperature in the calculation is set to be 300 K.
The marine and land areas for the horizontal calculation were set to 300 and 200 m, respectively (Figure 3).
Cycle conditions were as follows: after an initial 2-min hold at 50°C and 10 minutes at 95°C, the samples were cycled 40 times at 95°C for 15 sec and 60°C for 1 min. Baseline and threshold for cycle threshold (Ct) calculation were set manually with Sequence Detection Software version 1.4.
Baseline and threshold for Ct calculation were set automatically with the 7500 software v2.06.
Baseline and threshold for Ct calculation were set automatically with ABI Prism SDS 2.1 software.
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