Therefore, calculations of turnover with linear regression models are inevitably difficult to perform when induction systems are utilized.
The calculation of the turnover frequencies (TOFs) enables to show that, among all the samples studied, Ph-PMO-PrSO3H exhibit the higher catalytic activity.
Fig. S2 Topograph calculation of protein turnover rate and peak alignment for isotopomer abundance.
The concentration of Nox4 in cell lysates was estimated using Western blotting and allowed calculation of a turnover of ∼200 mol of H2O2 mol−1 (mof of Nox4)−1.
Calculation of the turnover rate (kcat) of the enzyme was not done due to the high level of recombinant glycosylation of CmINV, making accurate molecular weight determination misleading.
This is key to accurate calculation of protein turnover rates; other heavy isotope labeling approaches require complex calculations to indirectly estimate and compensate for changes in precursor pool enrichment (Price et al., 2010, 2012; Kim et al., 2012).
The Topograph software program was developed for the deconvolution and measurement of peptide isotopologue abundances from LC-MS chromatograms, and the calculation of peptide turnover rates, as previously described (18) (http://proteome.gs.washington.edu/software/topograph/).edu/software/topograph/
The topograph software program (http://proteome.gs.washington.edu/software/topograph/)(http://proteome.gs.washington.edu/software/topograph/) was developed for the measurement of peptide isotopologue abundances from LC-MS/MS chromatograms and the calculation of peptide turnover rates (Hsieh et al., 2012).
Using peptides attached with a fluorescent label allows detection and quantification of both forms and hence the calculation of the reaction turnover.
It involves the calculation of three indices: similarity, turnover and diversity as well as the detailed analysis of trajectories of change.
