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Therefore, the computational complexity, in unit of N t -dimensional correlation calculation, of template matching can be represented as O(N ϕ N σ ).
Calculation of template copy numbers was based on the "threshold cycle" (CT) at which each fluorescent signal was first detected above background.
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This threshold is also well matched to ab initio calculations of template library complexity relevant to low-level mutation calling in tumor specimens.
This method enables calculation of the starting template concentration and is therefore a frequently used analytical tool in evaluating DNA copy number, viral load, SNP detection, and allelic discrimination.
Standard curve generated by TSR control template allows the calculation of the amount of template with telomeric repeat created by telomerase using 7000 SDS system software (Applied Biosystem).
This requires additional disk space for on-instrument storage, and will increase the waiting time for calculation of the cluster position template.
In this paper,the traditional Sobel edge detection algorithm for the calculation of the gradient of less template and the multiplication operation is time-consuming, resulting in complex image edge detection is not clear and the slow processing speed,this paper puts forward a method to increase the gradient template and change the way the new multiplication gradient.
The pattern match decoder also includes divisive normalization in the calculation of similarity between responses and templates, however it has no influence on the decoder's output because it uses a winner-take-all operation and the normalization factor is identical for all candidate templates.
A derivation of Eq. 1 from PET nomenclature is provided as supplementary information (Additional file 2), as is a template spreadsheet for calculation of ftb (Additional file 3).
To facilitate implementation of the models in practice, a spreadsheet template for the calculation of the conditional model and SAS programs for the Poisson process are posted on the first author's website.
Homology-based calculation of Colias PEPCK structures using these templates was done with MODELLER 9.8 [ 19]; in each case, 5 replicates were run and the best-scoring one (i.e. with lowest value of the molpdf criterion [ 19]) was used.
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