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The homology of VGSCs was calculated by aligning sequences using ClustalW [24] followed by calculation of sequence identity using GeneDoc [25].
We improve the GRA by introducing variation rate into the calculation of sequence difference, which enhances the correlation degree between indicators.
Presence of multiple transmembrane domains was predicted using TMHMM server v2.0 (http://www.cbs.dtu.dk/services/TMHMM/). Multiple alignments and calculation of sequence identities and similarities were made using GeneDoc software (http://www.nrbsc.org/gfx/genedoc/ebinet.htm).htm
The most time consuming is the estimation of the binding free energy, which for large interfaces and protein complexes requires intensive and long computational times, and the sequence search and calculation of sequence profiles for evolutionary-based measures.
This parallelism is performed distributing the similarity calculation of sequence pairs to available slaves.
This modelling reduces alignment errors around low-complexity regions separated by short spacers and affects the calculation of sequence similarity.
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Calculation of sequences of 'elementary symmetric functions' σ i, 1, σ i, 2, σ i, 3, ⋯, σ i, v i Open image in new window from s i, where each σ i, u i = ( σ i, u i, 1, σ i, u i, 2, σ i, u i, 3, ⋯, σ i, u i, r ′ ) Open image in new window, for 1 ≤ u i ≤ v i and 1 ≤ i ≤ t. 3.
Calculations of sequence similarity and the informativeness of sequences was done using the T-Coffee package [43].
The "n" row indicates number of PCR fragments included in the probabilistic calculations of sequence numbers.
Both background and procedures of the phylogenetic analysis are described in Additional File 1, alignments are shown in Additional Files 2, 3, 4 and 5, calculations of sequence identities are given in Additional Files 6 and 7, and phylogenetic trees are shown in Additional Files 8, 9, 10, 11 and 12.
For each simulated sequence set, ClustalW v2.0.10 [ 34] was used for calculation of multiple sequence alignments.
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