Your English writing platform
Discover LudwigExact(11)
The concentration of each plasmid was calculated based on absorbance at 260 nm, and a dilution series produced for calculation of copy number via qPCR.
Then, the concentration of P. putida KT2440 DNA was converted to a genome equivalent for calculation of copy numbers in the real-time PCR assays (Cottyn et al. 2011).
As an example of the type of genome-based information that can be obtained, we presented the successful calculation of copy number alterations and mutations from snap frozen as well as from FFPE breast tumor tissue.
Calculation of copy numbers (copy no).
Integrated densities used for the calculation of copy numbers were obtained using ImageJ software.
(i) Calculation of copy number profiles is mainly done as described in our previous publication (Boeva et al., 2010).
Similar(49)
We used 0.5 copies/haploid genome in our calculations of copy number of transgenes in the materials tested except for samples T25 and Bt11.
Otherwise, it is possible that too many partition will get multiple copies preventing an accurate calculation of the copy number of your miRNA.
Since the calculation of the copy number concentration includes dividing the copy number estimate by the assay volume, knowing the correct partition or droplet volume is a key factor when measuring DNA concentrations using digital PCR [ 29].
Calculation of gene copy number for each sample was done using the Copy Caller Software V1.0 (Applied Biosystems).
ACT1 gene was used as the standard for calculation of rDNA copy number.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com