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Calculation of conservation of CICPs within the alignments is calculated per alignment position by summing up the CICP occurrences per column and dividing by the total number of sequences that participated in the CICP study for that alignment.
ConSurf [ 62, 63] was used for calculation of conservation.
Visualization of alignments and calculation of conservation scores was done with Jalview v 2.8 [ 62].
For calculation of conservation ratios (see below), the proteomes of the following additional species were downloaded: Oryza sativa (Osa), Zea mays (Zma), Triticum urartu (Tur), Sorghum bicolor (Sbi), Hordeum vulgare (Hvu), Brachypodium distachyon (Bdi), and Aegilops tauschii (Ata).
For the calculation of conservation ratios, the E-values were averaged among group A (AM-competent dicots: Mtr, Gma, Sly, Stu, Vvi, Ptr); group B (AM-competent monocots: Osa, Zma, Tur, Sbi, Hvu, Bdi, and Ata); and group C (non-mycorrhizal dicots: Ath, Aly, Bra).
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Our workflow uses a modified version of the AL2CO software for calculation of positional conservation [ 38].
We applied AL2CO software for calculation of positional conservation to mask the generated optimal alignment [ 38].
In order to illustrate the calculation of the conservation score, we consider the example shown in Figure 8.
Calculation of a conservation score is similar to positional Shannon entropy evaluation, which is further normalized to scale from 0 to 100.
Calculations of water conservation and energy consumption as a result of improvement demonstrates the complexity of the balance between energy and water efficiency.
The relative error for the filtered density can reach up to 40%, and this uncertainty can propagate and contaminate calculations of the conservation equations.
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