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(B ) Calculation of colocalization coefficients in (A ).
(D ) Confocal imaging and (E ) calculation of colocalization coefficient of Ptch1-GFP and Rab7-RFP in Kif3a−/− and control MEFs.
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(A ) Confocal images showing colocalization of Ptch1-GFP or Δ2PY (green) with Rab7-RFP (red), and (B ) calculation of the colocalization coefficients in (A ) in transfected MEFs.
(A ) Confocal images showing colocalization of exogenously expressed Ptch1-GFP or Δ2PY (green) with native Cav-1 (red) at the rim of the plasma membrane, and (B ) calculation of the colocalization coefficients in (A ) in transfected MEFs.
All acquisitions, SIM calculations and measurements of colocalization were performed in the Zeiss Zen software (v. 11) (Zen Blue version, Carl Zeiss Microscopy).
For colocalization analysis, the gain was adjusted for each cell and degree of colocalization calculated using Pearson's correlation coefficient.
In a different approach, Costes and coworkers [3] proposed an automated method for segmentation, the calculation of modified Manders coefficients and a statistical validation of colocalization by a combination of block scrambling and probability density functions [3].
Calculation of Li's intensity correlation coefficient confirmed a high level of colocalization between αSyn and LC3, which, however, was significantly reduced for αSyn-A30P (αSyn-WT: 0.475±0.003; αSyn-A30P: 0.448±0.002; αSyn-A53T: 0.478±0.002).
Increased F in)/F out) ratio indicates higher degree of colocalization post axotomy; see Figure 7 figure supplement 1A and 'Materials and methods' for calculation of F in)/F out).
A calculation of the similarity scores showed a significant colocalization of cofilin with the mitochondria and HSC70.
b, e Lack of colocalization between VEGFR-2 and CD31.
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