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Decreasing the index until reaching Max H/3 and setting the corresponding intensity level as the initial estimation (Tcalcest) for calculation of calcium threshold (Tcalc).
(2) Calculation of the maximum histogram value (Max H ). (3) Decreasing the index until reaching Max H /3 and setting the corresponding intensity level as the initial estimation (T calcest) for calculation of calcium threshold (T calc).
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These parameters control the calculations in the calcium channel stochastic "open"/"close" dynamics, the calculation of the calcium ion concentration detected by the vesicle's "calcium sensor," and finally the calculation of the postsynaptic conductance at the afferent neuron in response to airborne sound fluctuations.
We have the advantage with the use of the absorbance spectrum between 500 and 600 nm, used in the calculation of intracellular calcium, that this is also sensitive to the presence of deoxy- or oxygenated myoglobin [ 7].
Calculation of the calcium threshold (Tcalc) for DR > 0.7 ∗imMax (brighter images) by (1) and Tcalc for DR < 0.7 ∗imMax was calculated by (2): (1) T calc = T calcest + im Max − T calcest ∗ 0.5, (2) T calc = T calcest + im Max − T calcest ∗ 0.2.
(5) Calculation of the calcium threshold (T calc) for DR > 0.7 ∗ imMax (brighter images) by (1 ) and T calc for DR < 0.7 ∗ imMax was calculated by (2 ): (1) T calc = T calcest + im Max − T calcest ∗ 0.5, (2) T calc = T calcest + im Max − T calcest ∗ 0.2.
Although the formation of other ions pairs was not taken into consideration for computation of activity of calcium ions, preliminary calculations showed that a small fraction of calcium ions are tied up as complexes and therefore do not affect the broad conclusions drawn above.
Sodium (Na) and potassium (K) were analyzed by flame photometric method, calcium (Ca) by EDTA titration, magnesium (Mg) by calculation after EDTA titration of calcium and total hardness, chloride (Cl) by argentometric titration.
16 When the individual did not have a calcium intake from diary but used supplements, calcium intake was classified according to the calculation of the amount of calcium contained in the supplement.
The calculation of total to ionized calcium ratio proved to be a better parameter for citrate accumulation than anion gap.
Dietary data were assessed using specially designed semiquantitative food frequency questionnaire calculations based on the daily intakes of calcium, carbohydrates, fats, and proteins.
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