The pour plate method was applied for calculation of total count of bacteria and the coliforms were enumerated by a violet red bile agar (VRBA) plate and brilliant green lactose bile broth (BGLB).
The CFU data obtained at various time points allow calculation of the number of bacteria bound, internalized, and killed.
The calculation of effective information of bacteria is more complex than viruses' because the genes may express more than once in the growth of bacteria.
The reliability of a commercial "viability" staining assay (Molecular Probes) is discussed based on the corresponding product information sheet: (I) Staining principle; (II) Concentrations of bacteria ; (III) Calculation of live/dead proportions in vitro.
The following formula was used for calculation of the number of stained bacteria per milliliter: mean bacterial count in one field X (filtration area size/field size) X (1/dilution factor × 0.5 ml).
Analysis and quantification software was developed to provide enumeration of internalized, bound external and total bacteria per cell, and calculation of mean bacteria per cell and fraction of bacteria internalized within each microplate well.
which is the proportion of the total reduction in Malthusian fitness of bacteria i by phage a (the same calculation applies for Ω bi ).
In general, handling of the flow chamber caused removal of a non-negligible number of bacteria, which was corrected for in the calculation of removal percentages.
MIC assays with 1, 2, and 3 were performed as explained in the MBC calculation protocol, according to recommendations from the CLSI for aerobic growth of bacteria.
The number of bacteria expressed as Colony Forming Units per gram (CFU/g) was then determined by calculation, considering the factors of dilution.
