Sentence examples for calculating the staining from inspiring English sources
Richardsen et al. [ 16] assessed COX-2 expression by calculating the staining index SI defined as a weighted sum of percentage fractions of cells showing various intensity of staining calculated by multiplying the staining intensity (graded 1 3) by the percentage of positively stained cells.
and processed to calculate the staining intensity and percent label for each sample.
We utilized proportion of protein-expressing cancer cells and staining intensity to calculate the staining index representing the protein expression.
The algorithm for determining the intensity of membrane-specific staining provided by the manufacturer was optimized and used to calculate the staining intensity and percent target labeled for each sample by digitally analyzing the color intensity.
To calculate the staining intensity of SIRT1 in the nucleus, mean pixel intensities were obtained for the nuclear region (∼10 cells) using NIS Element AR 3.0 software and these were averaged to generate a mean value.
Single-stranded labeled amplified products were hybridised to the arrays and a signal intensity value was determined for each spot on the array by calculating the quantitative staining value using IconoClust software (version 2; CLONDIAG).
In the case of KI-67 protein expression, the system was optimised for nuclear staining, calculating the percentage of stained nuclei.
The surface staining (S) and the surface of the nuclei (N) were determined for each image, and the area of staining then was normalized by calculating the ratio of surface staining to nuclei staining.
The relative expression to the staining with the isotype control was calculated by calculating the ΔMFI MFII antibody stained - MFI control antibody).
Briefly, software calculated the nuclear staining intensities for the proteins of interest in individual cells.
To quantify the number of ALP-positive cells and the total number of cells and calculate the positive staining rate, 10 non-overlapping fields of view were randomly selected under a light microscope.
