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All samples were tested in triplicate PCR reactions and the mean of the reactions was used for calculating the expression levels.
After calculating the expression levels of BAX relative to BCL-2 based on β-actin expression, we found that there was a >4.0 4.3-fold >4.0 4.3-foldhe BAX/BCL-2 ratincreaseSCs after exposure to phthalate esters compared winh the control treatment using DMSO.
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After calculating the expression level of each mapped unigene, a total of 1,060 unigenes were detected that had levels of expression that were significantly different between the drought-treated and control libraries.
For densitometric analyses, we calculated the expression levels of AR and ERα by dividing these values with that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), as the internal control.
Only reads that aligned to a unique location in the genome were used to calculate the expression levels.
To identify genes with different expression levels, we used the RPKM method (Reads Per kb per Million reads) to calculate the expression levels of the unigenes.
The RNA-seq mapping performed to calculate the expression levels of the assembled transcripts in both analyzed organs mapped the majority of paired-end reads (Table 5).
A robust multi-array average (RMA) algorithm was applied to calculate the expression levels, and a multiscale adaptive search (MAS) algorithm was used to calculate the detection calls.
To obtain the expression differences between sexes, we calculated the expression levels of mixed-sex, male and female adult worms based on both transcriptomic and proteomic analyses.
To calculate the expression levels of CEP genes, RNA-Seq reads were remapped to their reference genomes using the short-read aligner SMALT [ 75] with default settings.
To begin to understand the dynamics of TEs in A. aegypti we calculated the expression levels of transcripts derived from TEs through development.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com