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The same procedure is performed on both the sample (IS) and background (I0) images prior to calculating absorbance (eq 4).
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A close correlation (68 95%) between the measured and calculated absorbance was found.
The calculated absorbance spectra based on the time-dependent density functional theory (TD-DFT) shows very close similarity with the measured behaviours under UV light.
Later, the standard calibration (concentration vs absorbance) curves were automatically developed by the machine software from the inputted concentration values of reference samples and calculated absorbance values of solution for different metals.
Thus, Finlayson and Schaefer applied logarithmic preprocessing to obtain a hue parameter that was invariant to brightness and gamma [14], while van der Laak et al calculated absorbance for transmitted light microscopy images prior to determining a hue parameter [15].
Background spectra originated from pure water are collected immediately after each sample to calculate absorbance units as described below.
The absorbance was calculated as follows for each standard and sample solution: a calibration curve was prepared using the calculated absorbance for the catechin solution, and the total procyanidin in each test solution was calculated from the calibration curve.
DNA purity was determined by calculating the absorbance A260/A280 ratio.
Polysaccharide contamination was assessed by calculating the absorbance ratio at A260/230 nm [ 34].
DNA purity was determined by calculating the absorbance ratio at A260/280 nm.
The result of calculating the absorbance of each group is shown as a percentage over that of the untreated group.
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