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Fat-free mass, fat mass, and the percentage of body fat mass were calculated with validated equations [ 23] and in accordance with the National Institutes of Health guidelines [ 24].
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The serum level of calculated free testosterone (cFT), expressed in nanomoles per liter, was calculated with the validated equation of Vermeulen et al. 19 Statistical analyses were performed using the STATISTICA 9.1 data analysis software system (StatSoft, Tulsa, OK, USA).
Patient-specific organ and tissue doses were calculated with a validated Monte Carlo program.
30 Bioelectrical impedance analysis was performed with a four polar single frequency device (RJL Systems, Model 101A; Detroit, MI, USA) and percentage body fat calculated with a validated formula for children in this age group.
We defined an unfavourable prognosis for natural conception as a probability of natural conception within the next 12 months of less than 30%, as calculated with the validated synthesis model of Hunault.
SEP scores of validating patients were calculated with the 60-gene profile of the validated dataset.
Effectiveness was assessed using DAS28 excluding patient's global health component calculated with erythrocyte sedimentation rate DAS28-3 ESRR), a validated disease activity measure.
HOMA2-IR was calculated with serum glucose and C-peptide values using the validated calculator (accessed at http://www.dtu.ox.ac.uk).ac.uk
The genotypic score calculated with these six mutations was significantly associated with virological failure (OR = 4.1 for a difference of one mutation, CI95% [2.4; 7.0]; p < 10-4; cross-validated OR = 4.9).
The Pearson χ test was calculated with R. German strain DE9686 (B P1.7 2,4 F1 5:ST42 MT19) isolated in 2004 from a patient of IMD in Aachen was used as the target strain in a validated serum bactericidal assay (28 ).
Limiting comparisons to studies that calculated energy expenditure with validated questionnaires and that used the lowest quintile approach seems likely to ensure that at least the populations identified are similar.
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