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Specific activity was calculated with the level of total proteins in a lysate as determined.
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For the analyses in comparisons between patient subgroups and the control group, the odds ratios needed for detecting differences in genotype distributions were calculated with the power level of 0.8.
The percentages of diagnosed diabetes at 12 and 24 months after the inclusion in the study will be calculated and the level of association with exposure to the educational intervention, considering the potential confounders, will be assessed.
The ROC curve for subjective ultrasonographic assessment was calculated with the use of four levels of diagnostic confidence ("probably benign", "uncertain, finally classified as benign", "uncertain, finally classified as malignant" and "probably malignant").
Sample size of 90 was calculated with α level of 0.05 and β power of 0.8 for this study.
The level of expression was calculated with the crossing point (Cp) method implemented by the Roche LightCycler 480 software version 1.5.
Optimal geometry of decamer (later in this text designed as 10_opt) was calculated with DFT on the level of B3LYP hybrid model and 6-31G(d) polarization basis set [44].
The frailty index hazard ratios (HR) are calculated with % levels of the index (i.e. the HR measures a change of 0.01 on the index).
The standard error of measurement (SEM) with 95% CI was calculated to express the level of error in original and clinically meaningful units.
The sample size was calculated with a precision level of 3%, population size of 6 million, 95% Confidence Interval, and estimated asthma prevalence of 13%.
The sample size for the HELENA-CSS was calculated with a confidence level of 95% and ± 0.3 error, based on the variance in body mass index, and yielded a sample size of 300 in each city.
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