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Dye ratio was calculated with microarray analysis function on the Nanodrop-1000 (Thermo).
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Hybridization intensity data were extracted from the scanned array images, and the designations for presence or absence were calculated with Affymetrix Microarray Suite (MAS) 5.0 software.
After hybridization and scanning, probe cell intensities were calculated with the Affymetrix Microarray Analysis Suite (MAS version 5.0) [ 55].
Statistical differences in cytokine levels were calculated with significance analysis of microarrays (SAM [ 25]), and the SAM-generated results with a false discovery rate (FDR) of less than 10% were selected.
The correlation coefficients were first calculated with the signal intensities of all clones on the microarray slides.
Percentage values are calculated with respect to the total number of probes in the microarray platform used in each study.
For each microarray, the mean of those standard deviations was calculated with the data from spots with a background-corrected reference channel intensity less than 150 filtered out.
Fold changes were calculated with respective efficiency-corrected normalized quantities in the same fashion as for microarray data.
For the microarray data, the mean expression and fold changes were calculated with FDR (Benjamini Hochberg) multiple testing corrections using ArraySTAR software (3801 Regent Street Madison, WI53705, USA).
Differential expression was confirmed for all genes and the log2 fold change) calculated with the qRT-PCR data consistently showed a greater magnitude of change compared to the log2 fold change) calculated with the microarray data.
Correlation between DNA microarray M' and appropriate M = log2 optical densitystress-exposed*optical densitycontrol-1) values for Northern blot autoradiography pictures was calculated with Microsoft Excel 97 software.
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