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The B index was calculated using the program Skew Calculator 2003 (http://www.eeb.ucla.edu/Faculty/Nonacs/shareware.htm).htm
The cell doubling time was calculated using the program from the Doubling Time Online Calculator http://www.doubling-time.com/compute.php.php
The curves were calculated using the program CURVES [30].
Nucleotide distances were calculated using the program DNAsp [41] and the program "Distmat" included in EMBOSS package [40].
Activities and saturation indexes of the solutions were calculated using the program PHREEQC [60] and the database Phreeqc.
Amplification efficiencies and threshold cycle (Cq) values were calculated using the program LinRegPCR (Ruijter et al. 2009).
Biomass of biofilms was calculated using the program COMSTAT [51].
Power was calculated using the program Quanto (http://hydra.usc.edu/GxE/).
Measures of allelic richness (AR) were calculated using the program Fstat version 2.9.3.2 [40].
Nei standard genetic distances were calculated using the program Microsat (http://hpgl.stanford.edu/projects/microsat/).edu/projects/microsat/
The r coefficient was then calculated using the program SPAGeDi 1.0 [34].
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