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In the prediction process, the RBS scores for all candidate genes were calculated using the constructed PWMs and the frequency distributions of the positions.
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For each sample the level of the individual antiviral drugs was calculated using the calibration line constructed on the same day.
We compared the prevalence of chronic conditions and current smoking among women in major cities and contrasting cities. Weighted prevalence was calculated using the sampling weight factor constructed by RAND (27) to allow use of the dataset for cross-sectional analysis.
Criterion validity was calculated using the Spearman's correlation, construct validity using the Spearman's correlation and the Mann-Whitney test, and the magnitude of the difference between groups using effect size (ES).
a. ACE z values were calculated using the Translation40 gene set to construct f o and all ORFs to construct f n.
All codon statistics were calculated using the Translation40 gene set to construct f o and all ORFs to construct f n. 2. Average divergence (dS) and correlation were measured among putative orthologs with dS < 1.5 between reference and target genomes.
The copy numbers of mtDNA and nDNA were calculated using the calibration curves that were constructed from dilution series of recombinant plasmids.
All codon statistics were calculated using the Translation40 gene set to construct f o and the shared set of ORFs to construct f n. b.
A DNA distance matrix was calculated using the F84 method, and trees were constructed by UPGMA (Unweighted Pair Group Method with Arithmetic Mean) and neighbor-joining methods [ 60, 61].
Relative expression of nucA is based on the ratio of the nucA transcript versus the reference gene transcript (gyrA), in cultures with cells containing the specific nucA construct, and was calculated using the relative expression software tool (REST) [ 65].
All the networks were constructed and the parameters calculated using the software Cytoscape [ 51] and its network analyzer tool.
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