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The mitochondrial tree shown in Figure 2D was significantly rejected by the KH test, if its likelihood value was calculated using nuclear DNA-coded genes; its log-likelihood difference from the ML tree was 34.9 ± 18.0 (P-value = 0.03) and 42.3 ± 20.8 (P-value = 0.02) in concatenated alignment analysis and totalml analysis, respectively.
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They pedalled stationary bikes until they were exhausted, and had their lean body mass calculated using a nuclear counter at Los Alamos.
Estimates of genetic diversity were calculated using both nuclear and chloroplast genetic data.
The composition of the copolymers has been calculated using 1H nuclear magnetic resonance (n.m.r).
YFP fluorescence intensity was calculated for nuclear and whole cell area and the percentage nuclear fluorescence calculated using the equation: percentage nuclear fluorescence = (nuclear intensity/whole cell intensity) × 100.
The percentage nuclear fluorescence was calculated using the equation: percentage nuclear fluorescence = [(nuclear intensity-background)/(whole cell intensity-background)] × 100.
Samples were detected in triplicate and relative expression levels were calculated using U61 small nuclear RNA (SNORD61, Qiagen, Valencia, CA) as the endogenous control.
Mitochondrial area was calculated using the plasma membrane and nuclear stains to delineate the cytoplasm and MTR to stain the mitochondrial population.
Labeling index (percentage of positively stained cells) is calculated using 2 equations based on nuclear versus cytoplasmic/membrane location of signals.
Volumes of chromosome territories and total nuclear volumes were calculated using surfaces feature of Imaris Software version 7.1.1 (Bitplane St. Paul, MN).
The expression of each miRNA was normalized to U6 small nuclear RNA and calculated using the method.
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