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Otherwise, fluorescence intensity of DCF-DA was measured and calculated using flow cytometry (Becon Dickinson, Franklin Lakes, NJ, USA).
Percentage of cells with bound mycoplasmas and mean fluorescence intensity (MFI) were calculated using flow jo software [ 24].
Response: "The linear dependence of the flow profile was calculated using flow models from the original manuscript describing this chamber (Usami, Chen et al. 1993).
After 10 days of co-culture, adherent and nonadherent cells were collected, and the number of the cells was calculated using flow cytometry and a cell counter (Fig. 4A lower panel).
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Blood counts were calculated using Flow-Count Fluorospheres (Beckman Coulter).
The residence time is calculated using solid flow or liquid flow model, depending on the degree of decrosslinking.
Blood flow (in ml/min) was then calculated using mean flow velocity × cross-sectional area [CSA; where CSA = π × (mean diameter/2) and blood flow = time-averaged mean flow velocity × CSA × 60].
Reviewer #1: 1) Flow chamber: a) Critique: Please explain what is the actual flow profile It is unclear if the linear dependence shown in Figure 1 was obtained experimentally or calculated using laminar flow models.
Reactive hyperemia was calculated using the flow change as a percentage of the baseline.
It is unclear if the linear dependence shown in Figure 1 was obtained experimentally or calculated using laminar flow models.
bThe purity of the CD5+/-IgM + cells was calculated using a flow cytometer based at the Brain Sciences Institute.
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