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Predicted values were calculated using published reference values [13].
Means were calculated using these values.
The temperature was then calculated using the obtained ADC value to a value in degrees celsius using equation 2 below.
In order to identify WGD events, the evolutionary distances of homologous gene pairs are often calculated using synonymous mutation or 4DTV values, and the histogram of values interrogated for distinct peaks (Tuskan et al. 2006; Barker et al. 2008).
Note: All values calculated using kappa statistics.
M-values and absolute methylation values were calculated using minfi.
Calculated logP values are indicated (calculated using the Molinspiration molecular property calculation services at www.molinspiration.com/cgi-bin/properties).
p-values calculated using Mann-Whitney U-test.
P value calculated using Kruskal Wallis test.
P- value calculated using one-way ANOVA.
b P value calculated using χ test.
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