(B) Relative expression of ALK was calculated using spot densitometry on total protein immunoblots of MDA-MB231 and MCF12A cells.
(B) Relative expression of ALK was calculated using spot densitometry on cytosolic and nuclear immunoblots of MDA-MB231 and MCF12A cells.
dsRNA concentration was then calculated using gel electrophoresis and densitometry analysis (ImageJ).
Protein values were calculated using the Aida 2D-Densitometry software (Raytest, Straubenhardt, Germany).
The densitometry was calculated using ImageJ.
Densitometry was calculated using ImageJ (Wayne Rasband; National Institute of Mental Health, Bethesda, MD, USA).
Images were obtained and densitometry was calculated using Odyssey application software version 3.0.21.
Densitometry calculations for western blot were calculated using the ImageJ software, verifying for non-saturation and subtracting background.
P-values for pAKT densitometry and prothrombin time were calculated using a two-tailed, unpaired t-test.
Through densitometry analysis, the electrophoretic mobility was calculated, using molecular weights of proteins with reference to protein marker standards (Priya et al. 2013).
