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The amount of ammonia liberated was calculated using ammonium chloride standard curve.
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Also, the Michael addition reaction rate constants (km) were calculated using microscopic acidic dissociation constant of ammonium groups in 4-aminobenzoic acid.
The concentration of ammonia was then calculated using a standard curve prepared with ammonium chloride.
The effect of various other parameters on ammonium flux was calculated using robustness analysis.
The objective of the model (in silico cell) was growth and the constraints were set to represent the environmental conditions and the flux through the ammonium exchange reaction was calculated using linear optimization at steady state.
The thermodynamic model was constructed and the solubility of malachite Cu2(OH)2CO3 in the ammonia-ammonium chloride-ethylenediamine(En -water systEn -waterlculated usystemhe exponential computation method based on both mass balance and charge balance.
Several key statistics were calculated using SLIDER.
Linear regression analyses were calculated using Excel.
Ct values were calculated using ABI software.
Statistical significance was calculated using R software.
C/N ratio was calculated from the measured value of C and N. Nutrients such as Na, K, and Ca were determined after extracting the sample using ammonium acetate method (Simard 1993) and analyzed by atomic absorption spectrophotometer.
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