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Protein concentrations were calculated using absorbance at 280 nm.
Data were expressed as the percent survival of the control, which was calculated using absorbance after correcting for background noise.
Total RNA concentration was calculated using absorbance at 260 nm (1 AU = 40 μg/mL), and all samples were dried to completion by SpeedVac and resuspended in ddH2O to a final normalized concentration of 10 ug/μL.
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Electron donor concentrations at start of linear phase of the decrease of absorbance were calculated using the absorbance coefficient (εNADH = 6300 M−1 cm−1; εNADPH = 6200 M−1 cm−1; εNRH-derivate = 4480 M−1 cM−1).
Analyte concentrations were calculated using the absorbance at 280 nm with the theoretical extinction coefficients.
Concentrations of the 3WJs were calculated using the absorbance of UV light at 260 nm using a Nanodrop 2000 (Thermo Scientific) using an optical density equaling one as 40 μg/mL and 50 μg/mL for RNA and DNA, respectively.
Confirmed peptide fractions were dried under vacuum, redissolved in water, and concentrations were calculated using the absorbance of Oregon Green 488 at 491 nm (Extinction coefficient 83,000 cm−1 M−1 in 50 mM potassium phosphate, pH 9).
IC50 values from yeast experiments were calculated using the absorbances of each treated transformant culture at the time when the corresponding untreated culture was closest to 0.5.
Cell viability was calculated using the formula: (absorbance of sample - absorbance of medium/ absorbance of vehicle control - absorbance of medium) x 100.
Percent viability was calculated using the formula (absorbance of treated cells)/ (absorbance of control cells) × 100.
Therefore, the molar velocity of liquid phase and components is calculated using carbon dioxide absorbance and reaction stoichiometry.
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