For all variables, within-group differences were calculated using a repeated-measures ANOVA, with all data obtained from all time points, and with time as the independent variable.
The sample size for the main RCT was calculated using a 20% baseline rate of repeat injury due to violence (based on a chart review of 4 months of visits to the sites) and an effect size of a 40% relative decrease (absolute 8% decrease) in repeat injury due to violence in the intervention arm.
Repeat density was calculated using a sliding window of 1 kb with a step size of 500 bp.
The interassay coefficient of variation of titers, expressed as log10 (calculated using an internal control repeated in 5 different assays, mean 2.56, standard deviation 0.35) was 13.67%.
WBs for each protein were repeated three times and the optical densities of the bands were calculated using a MiVnt image analysis system (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
GC content and repeat content analyses for 10 kb windows in the C. elegans genome was calculated using a combination of BEDTools and custom Perl and R scripts.
However, that total is calculated using a different Bitcoin price.
The validation parameters for species under study are presented in Table 2. Limits of detection (LODs) were calculated using the repeated analyses of the blank and the calculation algorithm presented by Huber.
Significance limits were calculated using ANOVA repeated measures or one way ANOVA followed by Student's paired t test for experiments with multiple time points or a single time point, respectively, except where indicated otherwise.
The changes in MMP/TIMP concentration between pre-treatment and follow-up samples were calculated using repeated measures ANOVA with dexamethasone as a covariate.
