Sentence examples for calculated the expression of from inspiring English sources
MiRProf calculated the expression of known miRNAs.
We calculated the expression of modules in different level in terms of cell cycles (or approximately reprogramming days).
We also calculated the expression of So0003, So0007, So0014, So0015, and So0025 relative to that of reference genes.
Finally, to more directly compare the qPCR results of the current study with those obtained previously [ 9], we calculated the expression of rh3, rh4 and rh6 relative to the total of these three rhodopsin mRNA (omitting the rh5 expression data).
To show the internal variability of TS, TS-L and NS samples we calculated the expression of proteins as percentage relative to the AK sample (set to 100%) which are the following: Fig 2D: TS = 106 ± 16%; Fig 2E: TS = 78 ± 4%; Fig 2F: TS-L = 39 ± 6%; supplementary Fig S1: NS = 906 ± 281%.
We used BEDTools [ 43] to isolate aligned reads that intersected the regions of interest in the sense direction and calculated the expression of these regions by dividing the number of reads in a region by the length of the region and the number of aligned reads and multiplying by 10.
The mean Ct values from duplicate measurements were used to calculate the expression of the target gene using the formula: fold change in gene expression, 2−ΔΔCt = 2−{ΔCt (SFN-treated samples)- ΔCt (untreated control)}, where ΔCt = Ct (hTERT)- Ct (GAPDH).
