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We calculated serum total lipids using an enzymatic summation method (Phillips et al. 1989).
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We calculated serum concentration of total lipids using the concentrations for triglycerides and total cholesterol measured by an enzymatic method: total lipids = (2.27 × total cholesterol) + triglycerides + 62.3 mg/dL (Phillips et al. 1989).
The mean ulcer index (MUI) was calculated and serum total anti-oxidant level determined.
Low density lipoprotein-cholesterol was calculated from serum total cholesterol, triglycerides (TG), and HDL-C, except when TG concentration was > 4.5 mmol/L [ 17].
The main outcome measure was description of the ACTH-cortisol response by calculating serum free cortisol and measuring total cortisol and ACTH concentrations.
We calculated total serum lipids by the formula total lipid = [total cholesterol (mg/dL) × 2.27] + triglycerides (mg/dL) + 62.3.
The serum OC concentrations were standardized by dividing by the calculated total serum lipid concentration.
We determined total cholesterol and triglycerides by enzymatic techniques, and calculated total serum lipid concentrations (Phillips et al. 1989).
In our previous study of the same population, we also analyzed data using cholesterol and triglycerides as separate covariates rather than using the calculated total serum lipids.
This resulted in only minor differences in results, and therefore in the present analysis we have used only the more traditional calculated total serum lipid values.
We calculated total serum lipids using the formula given in the NHANES laboratory manual for dioxins (CDC 2007c): Age (years), sex, race (Mexican American, African American, Caucasian, or other), and body mass index (BMI) were also included as covariates.
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