One noted the calculated physical preparation needed to set in higher gear the vocal demands of the part, yet nothing rang false.
The unloaded shortening velocity (VMAX) was calculated for each preparation according to Hill [ 20] using Sigma Plot 4.0 (Systat Software Inc., Richmond, California, USA).
The unloaded shortening velocity (V MAX) was calculated for each preparation according to Hill [ 20 ] using Sigma Plot 4.0 (Systat Software Inc., Richmond, California, USA).
Tension measurements were normalised to the cross-sectional area for each preparation, calculated assuming an elliptical cross section using the formula cross-sectional area = D1/2 × D2/2 × π, with D1 and D2 representing width and thickness, and expressed as tension.
Numbers of platelets in A-PRF and CGF preparations calculated by the indirect subtraction method are also shown in Fig. 1 (upper panel).
The amount of library required for template preparation was calculated using the template dilution factor calculation described in the protocol.
The mean OD ratio of PRBC-activated HLEC to RBC-activated HLEC was calculated for each PRBC preparation, using a positivity cut-off of 3, as recommended elsewhere [20], [26].
The contractile response for each preparation was calculated as stress (Newtons/m), normalized to the cross-sectional area of the longitudinal smooth muscle layer as previously described [ 9].
The relative antibacterial potency of a given preparation was calculated by comparing its zone of inhibition with that of the standard antibiotic tetracycline.
The minimum concentration of the sugar in the final reaction mixture, which completely inhibited 32 hemagglutination units of the protein preparation, was calculated.
