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Reagent cost calculations (rounded to the nearest pound) were calculated per sample and are based on standard operational procedures employed at the Wessex Regional Genetics Laboratory, average referral rates per annum, average detection rates per gene and reagent list prices as at December 2003.
Two effective moduli were calculated per sample, based on the deformations measured using the machine platens and a sample-mounted extensometer.
The mean, standard deviation, and coefficients of variations (CV) were calculated per sample.
Means±S.D. (technical) were calculated per sample measured, and S.D. propagated from normalization gene measurements to target gene measurements.
Each cDNA was analyzed in triplicate, after which the average threshold cycle (Ct) was calculated per sample.
Each cDNA was analyzed in quadruplicate, after which the average threshold cycle (Ct) was calculated per sample.
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The mean, standard deviation (SD), range, and coefficients of variation (CV) were calculated per measured sample using Microsoft Office Excel Microsoft Corporationn, Redmond, WA, USA) and SPSS for Windows (version 16.0, Chicago, IL, USA).
SCOUT detects rare deletions and duplications at each targeted site by initially calculating per-probe scores for each sample, using a one-component SCIMM model extended to describe fluorescence data for SNP heterozygotes (Mefford et al., 2009).
Summary shedding rates for oral, anogenital and plasma samples were calculated per participant (persons with at least one sample with HHV-8 detected/total participants) and per sample (swabs or plasma samples with HHV-8 DNA detected/total samples collected).
The genomic DNA was used in 3 different concentrations 2 ng, 1 ng and 0.5 ng and subsequently copies were calculated per genome for each sample.
Genes were functionally grouped (Hes6, Hes6-Chga, Hes6-Ddc, Ascl1, Ascl1-Hes6-Nts-Ddc) and a median expression intensity calculated per group for each sample.
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