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To predict amphiphilic structure of peptides, helical wheels models were calculated on the server [ 102].
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The cavity volumes, as calculated on the CASTp server, are, respectively, 123Å and 111 Å in the BidBH3mini BaxΔC26 and the BimBH3mini BaxΔC26, similar in size to the BidBH3 BaxΔC21 cavity of 140Å.
The user must upload the molecule structures and then all the necessary descriptors are calculated directly on the server side.
The predicted melting free energies of the 4 terminal base pairs of both 5' and 3' termini of the siRNA duplex were calculated based on the Sfold server algorithm (http://sfold.wadsworth.org/sirna.pl).org/sirna.pl
The genealogical sorting index (gsi) was calculated on both genome and TMP phylogenies using an online server (http://www.genealogicalsorting.org) [ 40].
All calculations are done on the server.
Free energies [ 37] and DNA structures were calculated based on the Mfold Web Server [ 38].
Interface areas were calculated with the PISA server [51].
The conservation scores at each amino acid position were calculated using the same server.
The degree of conservation was calculated using the ConSurf Server (http://consurf.tau.ac.il).
Accessible surfaces were calculated using the Proface server[ 82].
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